M-CSF/CSF1 Kit ELISA

N° du produit ABIN6720397

N° du produit (Fournisseur): koa0253

Détail du produit M-CSF/CSF1 Kit ELISA

Antigène: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

N° du produit (Fournisseur): koa0253

Fournisseur: Rockland

Fonction: Human M-CSF Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum, Tissue Lysates, Urine

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant human M-CSF. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application:

ELISA_Dilution: 62.5 pg/mL-4000 pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human M-CSF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, tissue lysates, serum, plasma (heparin or EDTA), saliva or urine to each empty well. It is recommended that each human M-CSF standard solution and each sample be measured in duplicate.

Commentaires:

Standard: Expression system for standard: E.coli, Immunogen sequence:  E33-S190

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Autre désignation: M-CSF (CSF1 Produits)

Sujet:

Synonyms: Colony stimulating factor 1, Colony stimulating factor 1 (macrophage), Colony stimulating factor macrophage specific, CSF 1, CSF-1, CSF1, CSF1_HUMAN, Csfm, Lanimostim, M CSF, M-CSF, Macrophage colony stimulating factor, Macrophage colony stimulating factor 1, MCSF, MGC41930, op, OTTHUMP00000013364, OTTHUMP00000013365, OTTMUSP00000032803, OTTMUSP00000032804, Processed macrophage colony-stimulating factor 1, RP11-195M16.2

Background: M-CSF, also called CSF1, has a role in development of the placenta. Uterine CSF1 concentration is regulated by a synergistic action of estradiol and progesterone. CSF1 is produced by uterine glandular epithelial cells. It had been found that FMS, the CSF1 receptor, is expressed in placenta and choriocarcinoma cell lines1. The CSF1 gene is mapped to 1p21-p13 and contains 10 exons and 9 introns spanning 20 kb2. And there are 2 forms of CSF1, with 224 and 522 amino acids, resulting from alternative splicing3.

ID gène: 1435

NCBI Accession: NP_000748

UniProt: P09603

Pathways: Signalisation RTK