Cathepsin D Kit ELISA
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- Antigène Voir toutes Cathepsin D (CTSD) Kits ELISA
- Cathepsin D (CTSD)
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Application
- ELISA
- Fonction
- Mouse Cathepsin D ELISA Kit.
- Type d'échantillon
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Specificité
- This ELISA antibody pair recognizes Mouse Cathepsin D.
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product CTSD Kit ELISA
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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- Antigène Voir toutes Cathepsin D (CTSD) Kits ELISA
- Cathepsin D (CTSD)
- Autre désignation
- Cathepsin D (CTSD Produits)
- Synonymes
- catD Kit ELISA, fb93e11 Kit ELISA, fj17b09 Kit ELISA, wu:fb93e11 Kit ELISA, wu:fj17b09 Kit ELISA, LOC398557 Kit ELISA, Cathd Kit ELISA, CatD Kit ELISA, LOC100136761 Kit ELISA, CTSD Kit ELISA, DKFZp469J0315 Kit ELISA, DDBDRAFT_0205785 Kit ELISA, DDBDRAFT_0215012 Kit ELISA, DDB_0205785 Kit ELISA, DDB_0215012 Kit ELISA, CLN10 Kit ELISA, CPSD Kit ELISA, CD Kit ELISA, cathepsin D Kit ELISA, cathepsin D S homeolog Kit ELISA, lysosomal aspartic protease Kit ELISA, cathepsin D-like Kit ELISA, ctsd Kit ELISA, LOC398557 Kit ELISA, ctsd.S Kit ELISA, CatD Kit ELISA, LOC5567565 Kit ELISA, LOC100136761 Kit ELISA, CTSD Kit ELISA, Cathd Kit ELISA, ctsD Kit ELISA, catd Kit ELISA, Ctsd Kit ELISA, LOC443060 Kit ELISA
- UniProt
- P18242
- Pathways
- Peptide Hormone Metabolism
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