Integrin beta 3 Kit ELISA

N° du produit ABIN6730101

Détail du produit Integrin beta 3 Kit ELISA

Antigène: Integrin beta 3 (ITGB3)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Integrin beta 3/CD61 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human Integrin beta 3.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Integrin beta 3 (ITGB3)

Autre désignation: Integrin beta 3 (CD61 (ITGB3 Produits)

Synonymes: BDPLT16 Kit ELISA, BDPLT2 Kit ELISA, CD61 Kit ELISA, GP3A Kit ELISA, GPIIIa Kit ELISA, GT Kit ELISA, INGRB3 Kit ELISA, integrin Kit ELISA, INTB3 Kit ELISA, GPIIIA Kit ELISA, cd61 Kit ELISA, gp3a Kit ELISA, itgb3 Kit ELISA, itgb3.2 Kit ELISA, itgb3.1 Kit ELISA, integrin subunit beta 3 Kit ELISA, integrin beta 3 Kit ELISA, integrin subunit beta 3 L homeolog Kit ELISA, integrin beta 3a Kit ELISA, integrin beta 3b Kit ELISA, ITGB3 Kit ELISA, Itgb3 Kit ELISA, itgb3.L Kit ELISA, itgb3a Kit ELISA, itgb3b Kit ELISA

UniProt: Q9Y6R9

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex