EpCAM Kit ELISA

N° du produit ABIN6730115

Détail du produit EpCAM Kit ELISA

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse EpCAM/TROP-1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse EpCAM.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Autre désignation: EpCAM (TROP-1 (EPCAM Produits)

Synonymes: DIAR5 Kit ELISA, EGP-2 Kit ELISA, EGP314 Kit ELISA, EGP40 Kit ELISA, ESA Kit ELISA, HNPCC8 Kit ELISA, KS1/4 Kit ELISA, KSA Kit ELISA, M4S1 Kit ELISA, MIC18 Kit ELISA, MK-1 Kit ELISA, TACSTD1 Kit ELISA, TROP1 Kit ELISA, ECS-1 Kit ELISA, ECS1 Kit ELISA, ESA1 Kit ELISA, M17S1 Kit ELISA, EGP Kit ELISA, Ep-CAM Kit ELISA, sb:cb6 Kit ELISA, tacstd Kit ELISA, wu:fj17g02 Kit ELISA, zgc:110304 Kit ELISA, zgc:77119 Kit ELISA, tacstd1 Kit ELISA, MGC80540 Kit ELISA, EPCAM Kit ELISA, egp Kit ELISA, ksa Kit ELISA, m4s1 Kit ELISA, mk-1 Kit ELISA, cd326 Kit ELISA, egp40 Kit ELISA, mic18 Kit ELISA, trop1 Kit ELISA, ep-cam Kit ELISA, hegp-2 Kit ELISA, co17-1a Kit ELISA, ga733-2 Kit ELISA, CD326 Kit ELISA, Egp314 Kit ELISA, EpCAM1 Kit ELISA, GA733-2 Kit ELISA, Ly74 Kit ELISA, Tacsd1 Kit ELISA, Tacstd1 Kit ELISA, gp40 Kit ELISA, epithelial cell adhesion molecule Kit ELISA, flotillin 2 Kit ELISA, epithelial cell adhesion molecule S homeolog Kit ELISA, EPCAM Kit ELISA, FLOT2 Kit ELISA, epcam Kit ELISA, epcam.S Kit ELISA, Epcam Kit ELISA

UniProt: Q99JW5