CD44 Kit ELISA

N° du produit ABIN6730117

Détail du produit CD44 Kit ELISA

Antigène: CD44

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse CD44 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse CD44.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: CD44

Autre désignation: CD44 (CD44 Produits)

Synonymes: CDW44 Kit ELISA, CSPG8 Kit ELISA, ECMR-III Kit ELISA, HCELL Kit ELISA, HUTCH-I Kit ELISA, IN Kit ELISA, LHR Kit ELISA, MC56 Kit ELISA, MDU2 Kit ELISA, MDU3 Kit ELISA, MIC4 Kit ELISA, Pgp1 Kit ELISA, AU023126 Kit ELISA, AW121933 Kit ELISA, AW146109 Kit ELISA, HERMES Kit ELISA, Ly-24 Kit ELISA, Pgp-1 Kit ELISA, CD44A Kit ELISA, METAA Kit ELISA, RHAMM Kit ELISA, XCD44 Kit ELISA, cd44 Kit ELISA, cdw44 Kit ELISA, cspg8 Kit ELISA, ecmr-iii Kit ELISA, hcell Kit ELISA, lhr Kit ELISA, mc56 Kit ELISA, mdu2 Kit ELISA, mdu3 Kit ELISA, mic4 Kit ELISA, mutch-i Kit ELISA, pgp1 Kit ELISA, CD44 molecule (Indian blood group) Kit ELISA, syndecan 4 Kit ELISA, CD44 antigen Kit ELISA, CD44 molecule (Indian blood group) S homeolog Kit ELISA, CD44 Kit ELISA, SDC4 Kit ELISA, Cd44 Kit ELISA, cd44.S Kit ELISA

UniProt: P15379

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling