S100A9 Kit ELISA

N° du produit ABIN6730139

Détail du produit S100A9 Kit ELISA

Antigène: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse S100A9 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse S100A9.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Autre désignation: S100A9 (S100A9 Produits)

Synonymes: 60B8AG Kit ELISA, CAGB Kit ELISA, CFAG Kit ELISA, CGLB Kit ELISA, L1AG Kit ELISA, LIAG Kit ELISA, MAC387 Kit ELISA, MIF Kit ELISA, MRP14 Kit ELISA, NIF Kit ELISA, P14 Kit ELISA, Mrp14 Kit ELISA, S100A9 Kit ELISA, MRP-14 Kit ELISA, p14 Kit ELISA, 60B8Ag Kit ELISA, AW546964 Kit ELISA, BEE22 Kit ELISA, Cagb Kit ELISA, GAGB Kit ELISA, L1Ag Kit ELISA, S100 calcium binding protein A9 Kit ELISA, S100 calcium binding protein A9 (calgranulin B) Kit ELISA, S100A9 Kit ELISA, S100a9 Kit ELISA

UniProt: P31725

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins