CSTB Kit ELISA

N° du produit ABIN6730146

Détail du produit CSTB Kit ELISA

Antigène: CSTB (Cystatin B (Stefin B) (CSTB))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse Cystatin B ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse Cystatin B.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: CSTB (Cystatin B (Stefin B) (CSTB))

Autre désignation: Cystatin B (CSTB Produits)

Synonymes: CST6 Kit ELISA, EPM1 Kit ELISA, EPM1A Kit ELISA, PME Kit ELISA, STFB Kit ELISA, ULD Kit ELISA, CSTB Kit ELISA, Cyb Kit ELISA, Epm1 Kit ELISA, Stfb Kit ELISA, MGC189005 Kit ELISA, pme Kit ELISA, cst6 Kit ELISA, epm1 Kit ELISA, stfb Kit ELISA, AA960480 Kit ELISA, ARABIDOPSIS THALIANA PHYTOCYSTATIN 6 Kit ELISA, ATCYS6 Kit ELISA, ATCYSB Kit ELISA, cystatin B Kit ELISA, LOC100008600 Kit ELISA, LOC100136235 Kit ELISA, CPI1 Kit ELISA, cystatin B Kit ELISA, cystatin B (stefin B) Kit ELISA, cystatin 14a, tandem duplicate 2 Kit ELISA, cystatin-B Kit ELISA, CSTB Kit ELISA, Cstb Kit ELISA, cstb Kit ELISA, cst14a.2 Kit ELISA, CYSB Kit ELISA, cpi1 Kit ELISA, LOC101123265 Kit ELISA

UniProt: Q62426

Pathways: Response to Water Deprivation