Nicastrin Kit ELISA

N° du produit ABIN6730247

Détail du produit Nicastrin Kit ELISA

Antigène: Nicastrin (NCSTN)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse Nicastrin/NCSTN ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse Nicastrin.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Nicastrin (NCSTN)

Autre désignation: Nicastrin (NCSTN (NCSTN Produits)

Synonymes: CG7012 Kit ELISA, Dmel\\CG7012 Kit ELISA, Nct Kit ELISA, Nic Kit ELISA, S5A Kit ELISA, ST96B Kit ELISA, agro Kit ELISA, dNct Kit ELISA, nic Kit ELISA, NCSTN Kit ELISA, DKFZp469O2418 Kit ELISA, APH2 Kit ELISA, 9430068N19Rik Kit ELISA, AA727311 Kit ELISA, Aph2 Kit ELISA, D1Dau13e Kit ELISA, Kiaa0253 Kit ELISA, mKIAA0253 Kit ELISA, nicastrin Kit ELISA, Nicastrin Kit ELISA, NCSTN Kit ELISA, nct Kit ELISA, ncstn Kit ELISA, CpipJ_CPIJ001617 Kit ELISA, Ncstn Kit ELISA, aph-2 Kit ELISA

UniProt: P57716

Pathways: Signalisation Notch, Neurotrophin Signaling Pathway