RAB6A Kit ELISA

N° du produit ABIN6730273

Détail du produit RAB6A Kit ELISA

Antigène: RAB6A (RAB6A, Member RAS Oncogene Family (RAB6A))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human RAB6A ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human RAB6A.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: RAB6A (RAB6A, Member RAS Oncogene Family (RAB6A))

Autre désignation: RAB6A (RAB6A Produits)

Synonymes: fb43c01 Kit ELISA, wu:fb43c01 Kit ELISA, zgc:55788 Kit ELISA, rab6 Kit ELISA, MGC53077 Kit ELISA, rab6b Kit ELISA, rab6a' Kit ELISA, MGC76176 Kit ELISA, LOC692966 Kit ELISA, RAB6 Kit ELISA, 2610028L11Rik Kit ELISA, AA419671 Kit ELISA, Rab6 Kit ELISA, RCO4-3 Kit ELISA, Rab6b Kit ELISA, RAB6A, member RAS oncogene family Kit ELISA, RAB6A, member RAS oncogene family S homeolog Kit ELISA, ras small monomeric GTPase Rab6 Kit ELISA, ras-related protein Rab-6A Kit ELISA, rab6a Kit ELISA, rab6a.S Kit ELISA, LOC692966 Kit ELISA, RAB6A Kit ELISA, Rab6a Kit ELISA

UniProt: P20340