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ROBO3 Kit ELISA

ROBO3 Reactivité: Souris Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730276
  • Antigène Voir toutes ROBO3 Kits ELISA
    ROBO3 (Roundabout, Axon Guidance Receptor, Homolog 3 (ROBO3))
    Reactivité
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Mouse ROBO3 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Mouse ROBO3.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product ROBO3 Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes ROBO3 Kits ELISA
    ROBO3 (Roundabout, Axon Guidance Receptor, Homolog 3 (ROBO3))
    Autre désignation
    ROBO3 (ROBO3 Produits)
    Synonymes
    HGPPS Kit ELISA, HGPS Kit ELISA, RBIG1 Kit ELISA, RIG1 Kit ELISA, CG14345 Kit ELISA, CT17158 Kit ELISA, Robo-3 Kit ELISA, Robo3 Kit ELISA, dRobo-3 Kit ELISA, DmelCG5423 Kit ELISA, CG5423 Kit ELISA, ROBO3 Kit ELISA, robo3 Kit ELISA, DKFZp468G0223 Kit ELISA, Rbig1 Kit ELISA, Rig-1 Kit ELISA, Rig1 Kit ELISA, Robo3a Kit ELISA, Robo3b Kit ELISA, cb503 Kit ELISA, roundabout guidance receptor 3 Kit ELISA, roundabout 3 Kit ELISA, robo3 Kit ELISA, roundabout guidance receptor 3 L homeolog Kit ELISA, roundabout, axon guidance receptor, homolog 3 (Drosophila) Kit ELISA, ROBO3 Kit ELISA, robo3 Kit ELISA, Robo3 Kit ELISA, LOC658818 Kit ELISA, robo3.L Kit ELISA
    UniProt
    Q9Z2I4
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