×
Pour une meilleure utilisation, nous vous conseillons d’activer Javascript dans votre navigateur.
ROBO3 Kit ELISA
ROBO3
Reactivité: Souris
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730276
Détail du produit ROBO3 Kit ELISA
(cache)
Antigène
Voir toutes ROBO3 Kits ELISA
ROBO3
(Roundabout, Axon Guidance Receptor, Homolog 3 (ROBO3))
Reactivité
Souris
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Mouse ROBO3 ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes Mouse ROBO3.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Top Product
Discover our top product ROBO3 Kit ELISA
Alternatives
(show)
Information d'application
(cache)
Indications d'application
Optimal working dilution should be determined by the investigator.
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
(cache)
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
(cache)
Antigène
Voir toutes ROBO3 Kits ELISA
ROBO3
(Roundabout, Axon Guidance Receptor, Homolog 3 (ROBO3))
Autre désignation
ROBO3 (ROBO3 Produits )
Synonymes
HGPPS Kit ELISA, HGPS Kit ELISA, RBIG1 Kit ELISA, RIG1 Kit ELISA, CG14345 Kit ELISA, CT17158 Kit ELISA, Robo-3 Kit ELISA, Robo3 Kit ELISA, dRobo-3 Kit ELISA, DmelCG5423 Kit ELISA, CG5423 Kit ELISA, ROBO3 Kit ELISA, robo3 Kit ELISA, DKFZp468G0223 Kit ELISA, Rbig1 Kit ELISA, Rig-1 Kit ELISA, Rig1 Kit ELISA, Robo3a Kit ELISA, Robo3b Kit ELISA, cb503 Kit ELISA, roundabout guidance receptor 3 Kit ELISA, roundabout 3 Kit ELISA, robo3 Kit ELISA, roundabout guidance receptor 3 L homeolog Kit ELISA, roundabout, axon guidance receptor, homolog 3 (Drosophila) Kit ELISA, ROBO3 Kit ELISA, robo3 Kit ELISA, Robo3 Kit ELISA, LOC658818 Kit ELISA, robo3.L Kit ELISA
UniProt
Q9Z2I4
Vus récemment
(cache)