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Stanniocalcin 2 Kit ELISA

STC2 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730290
  • Antigène Voir toutes Stanniocalcin 2 (STC2) Kits ELISA
    Stanniocalcin 2 (STC2)
    Reactivité
    • 3
    • 2
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human Stanniocalcin 2/STC-2 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Human Stanniocalcin 2.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product STC2 Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes Stanniocalcin 2 (STC2) Kits ELISA
    Stanniocalcin 2 (STC2)
    Autre désignation
    Stanniocalcin 2 (STC-2 (STC2 Produits)
    Synonymes
    STC2 Kit ELISA, im:6908722 Kit ELISA, stc2 Kit ELISA, wu:fd13c01 Kit ELISA, zgc:136650 Kit ELISA, MGC145691 Kit ELISA, STC-2 Kit ELISA, STCRP Kit ELISA, AW125853 Kit ELISA, Stc2l Kit ELISA, mustc2 Kit ELISA, stanniocalcin 2 Kit ELISA, stanniocalcin 2a Kit ELISA, STC2 Kit ELISA, stc2a Kit ELISA, stc2 Kit ELISA, Stc2 Kit ELISA
    UniProt
    O76061
    Pathways
    Hormone Activity, ER-Nucleus Signaling, SARS-CoV-2 Protein Interactome
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