Syndecan 1 Kit ELISA

N° du produit ABIN6730295

Détail du produit Syndecan 1 Kit ELISA

Antigène: Syndecan 1 (SDC1)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse Syndecan-1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse Syndecan-1.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Syndecan 1 (SDC1)

Autre désignation: Syndecan-1 (SDC1 Produits)

Synonymes: CG10497 Kit ELISA, CT27296 Kit ELISA, Dmel\\CG10497 Kit ELISA, Dsyn Kit ELISA, Syd Kit ELISA, Syn Kit ELISA, dSdc Kit ELISA, l(2)10608 Kit ELISA, sdc Kit ELISA, syd Kit ELISA, syn Kit ELISA, Syn1 Kit ELISA, cd138 Kit ELISA, synd1 Kit ELISA, xSyn1 Kit ELISA, syndecan1 Kit ELISA, Syndecan-1 Kit ELISA, XSyndecan-1 Kit ELISA, SDC1 Kit ELISA, CD138 Kit ELISA, SDC Kit ELISA, SYND1 Kit ELISA, syndecan Kit ELISA, AA408134 Kit ELISA, AA409076 Kit ELISA, Sstn Kit ELISA, Synd Kit ELISA, Synd1 Kit ELISA, syn-1 Kit ELISA, HSPG Kit ELISA, SYNDECA Kit ELISA, Syndecan Kit ELISA, Syndecan Kit ELISA, syndecan 1 Kit ELISA, Sdc Kit ELISA, sdc1 Kit ELISA, SDC1 Kit ELISA, Sdc1 Kit ELISA

UniProt: P18828

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Lipid Metabolism