TWSG1 Kit ELISA

N° du produit ABIN6730321

Détail du produit TWSG1 Kit ELISA

Antigène: TWSG1 (Twisted Gastrulation Homolog 1 (TWSG1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse TSG ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse TSG.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: TWSG1 (Twisted Gastrulation Homolog 1 (TWSG1))

Autre désignation: TSG (TWSG1 Produits)

Synonymes: TWSG1 Kit ELISA, TSG Kit ELISA, DKFZp469J051 Kit ELISA, 1810013J15Rik Kit ELISA, 9030422N06Rik Kit ELISA, AW552143 Kit ELISA, D17Ertd403e Kit ELISA, Tsg Kit ELISA, Twg Kit ELISA, fi23f01 Kit ELISA, tsg Kit ELISA, tsga Kit ELISA, twsg1 Kit ELISA, wu:fi23f01 Kit ELISA, twsg Kit ELISA, tsg1 Kit ELISA, tsgb Kit ELISA, ztsg1 Kit ELISA, twisted gastrulation BMP signaling modulator 1 Kit ELISA, twisted gastrulation homolog 1 (Drosophila) Kit ELISA, twisted gastrulation BMP signaling modulator 1a Kit ELISA, twisted gastrulation BMP signaling modulator 1 S homeolog Kit ELISA, twisted gastrulation BMP signaling modulator 1 L homeolog Kit ELISA, twisted gastrulation BMP signaling modulator 1b Kit ELISA, TWSG1 Kit ELISA, Twsg1 Kit ELISA, twsg1a Kit ELISA, twsg1.S Kit ELISA, twsg1.L Kit ELISA, twsg1b Kit ELISA

UniProt: Q9EP52