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Calcineurin B Kit ELISA
CAN
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730337
Détail du produit Calcineurin B Kit ELISA
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Antigène
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Calcineurin B (CAN)
Reactivité
Toutes les réactivités sur Calcineurin B Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Human Calcineurin B ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes Human Calcineurin B.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternatives
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Information d'application
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Indications d'application
Optimal working dilution should be determined by the investigator.
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes Calcineurin B (CAN) Kits ELISA
Calcineurin B (CAN)
Autre désignation
Calcineurin B (CAN Produits )
Synonymes
PPP3RL Kit ELISA, BcDNA:RH11383 Kit ELISA, CG4209 Kit ELISA, CNB Kit ELISA, Can Kit ELISA, CnB Kit ELISA, Dmel\\CG4209 Kit ELISA, canB-4F Kit ELISA, dCnB1 Kit ELISA, Cnb Kit ELISA, Cblp Kit ELISA, CaNB2 Kit ELISA, protein phosphatase 3 regulatory subunit B, beta Kit ELISA, Calcineurin B Kit ELISA, calcineurin B Kit ELISA, protein phosphatase 3, regulatory subunit B, beta Kit ELISA, protein phosphatase 3 regulatory subunit B, alpha Kit ELISA, protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type II) Kit ELISA, PPP3R2 Kit ELISA, CanB Kit ELISA, Cnb Kit ELISA, Ppp3r2 Kit ELISA, PPP3R1 Kit ELISA
UniProt
Q96LZ3
Pathways
Cellular Glucan Metabolic Process , VEGF Signaling
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