PLA2G1B Kit ELISA

N° du produit ABIN6730357

Détail du produit PLA2G1B Kit ELISA

Antigène: PLA2G1B (Phospholipase A2, Group IB (PLA2G1B))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse PLA2G1B ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse PLA2G1B.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: PLA2G1B (Phospholipase A2, Group IB (PLA2G1B))

Autre désignation: PLA2G1B (PLA2G1B Produits)

Synonymes: C133 Kit ELISA, CG4346 Kit ELISA, Dmel\\CG42237 Kit ELISA, Dmel_CG4346 Kit ELISA, PLA2 Kit ELISA, GB13351 Kit ELISA, bvPLA2 Kit ELISA, PLA2A Kit ELISA, PPLA2 Kit ELISA, sPLA(2)-IB Kit ELISA, Pla2a Kit ELISA, sPLA2IB Kit ELISA, PLA2G1B Kit ELISA, CG42237 gene product from transcript CG42237-RB Kit ELISA, phospholipase A2 Kit ELISA, phospholipase A2 group IB Kit ELISA, phospholipase A2, group IB, pancreas Kit ELISA, phospholipase A2 group IVA Kit ELISA, CG42237 Kit ELISA, PLA2 Kit ELISA, LOC100631065 Kit ELISA, Pla2 Kit ELISA, PLA2G1B Kit ELISA, Pla2g1b Kit ELISA, PLA2G4A Kit ELISA

UniProt: Q9Z0Y2

Pathways: Inositol Metabolic Process, VEGF Signaling