MANF Kit ELISA

N° du produit ABIN6730370

Détail du produit MANF Kit ELISA

Antigène: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse MANF ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse MANF.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Autre désignation: MANF (MANF Produits)

Synonymes: ARMET Kit ELISA, ARP Kit ELISA, 3230402M22Rik Kit ELISA, AA407711 Kit ELISA, AA408789 Kit ELISA, AA673178 Kit ELISA, Armet Kit ELISA, D17914 Kit ELISA, D18Mgi17 Kit ELISA, MANF Kit ELISA, RBM15B Kit ELISA, ARP-like Kit ELISA, CG7013 Kit ELISA, DmMANF Kit ELISA, Dmel\\CG7013 Kit ELISA, anon-EST:ParkEST188 Kit ELISA, arp Kit ELISA, armet Kit ELISA, im:6909481 Kit ELISA, zgc:153327 Kit ELISA, manf Kit ELISA, mesencephalic astrocyte derived neurotrophic factor Kit ELISA, mesencephalic astrocyte-derived neurotrophic factor Kit ELISA, RNA binding motif protein 15B Kit ELISA, Mesencephalic astrocyte-derived neurotrophic factor Kit ELISA, mesencephalic astrocyte-derived neurotrophic factor S homeolog Kit ELISA, MANF Kit ELISA, Manf Kit ELISA, RBM15B Kit ELISA, manf Kit ELISA, manf.S Kit ELISA

UniProt: Q9CXI5