PFDN5 Kit ELISA
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- Antigène Voir toutes PFDN5 Kits ELISA
- PFDN5 (Prefoldin Subunit 5 (PFDN5))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Application
- ELISA
- Fonction
- Human PFDN5 ELISA Kit.
- Type d'échantillon
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Specificité
- This ELISA antibody pair recognizes Human PFDN5.
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product PFDN5 Kit ELISA
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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- Antigène Voir toutes PFDN5 Kits ELISA
- PFDN5 (Prefoldin Subunit 5 (PFDN5))
- Autre désignation
- PFDN5 (PFDN5 Produits)
- Synonymes
- MM-1 Kit ELISA, MM1 Kit ELISA, PFD5 Kit ELISA, 1190001O17Rik Kit ELISA, 1700010A06Rik Kit ELISA, D15Ertd697e Kit ELISA, Eig1 Kit ELISA, wu:fa09c11 Kit ELISA, wu:fb38c05 Kit ELISA, zgc:100894 Kit ELISA, pfdn5 Kit ELISA, prefoldin subunit 5 Kit ELISA, prefoldin 5 Kit ELISA, prefoldin subunit 5 S homeolog Kit ELISA, PFDN5 Kit ELISA, Pfdn5 Kit ELISA, pfdn5 Kit ELISA, ANI_1_408074 Kit ELISA, pfdn5.S Kit ELISA
- UniProt
- Q99471
- Pathways
- Unfolded Protein Response
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