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PFDN5 Kit ELISA

PFDN5 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730387
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    PFDN5 (Prefoldin Subunit 5 (PFDN5))
    Reactivité
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    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human PFDN5 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Human PFDN5.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product PFDN5 Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes PFDN5 Kits ELISA
    PFDN5 (Prefoldin Subunit 5 (PFDN5))
    Autre désignation
    PFDN5 (PFDN5 Produits)
    Synonymes
    MM-1 Kit ELISA, MM1 Kit ELISA, PFD5 Kit ELISA, 1190001O17Rik Kit ELISA, 1700010A06Rik Kit ELISA, D15Ertd697e Kit ELISA, Eig1 Kit ELISA, wu:fa09c11 Kit ELISA, wu:fb38c05 Kit ELISA, zgc:100894 Kit ELISA, pfdn5 Kit ELISA, prefoldin subunit 5 Kit ELISA, prefoldin 5 Kit ELISA, prefoldin subunit 5 S homeolog Kit ELISA, PFDN5 Kit ELISA, Pfdn5 Kit ELISA, pfdn5 Kit ELISA, ANI_1_408074 Kit ELISA, pfdn5.S Kit ELISA
    UniProt
    Q99471
    Pathways
    Unfolded Protein Response
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