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ERAP1 Kit ELISA

ERAP1 Reactivité: Souris Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730389
  • Antigène Voir toutes ERAP1 Kits ELISA
    ERAP1 (Endoplasmic Reticulum Aminopeptidase 1 (ERAP1))
    Reactivité
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Mouse Aminopeptidase PILS/ARTS1 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Mouse Aminopeptidase PILS.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes ERAP1 Kits ELISA
    ERAP1 (Endoplasmic Reticulum Aminopeptidase 1 (ERAP1))
    Autre désignation
    Aminopeptidase PILS (ARTS1 (ERAP1 Produits)
    Synonymes
    MGC166160 Kit ELISA, DKFZp469C044 Kit ELISA, ARTS-1 Kit ELISA, A-LAP Kit ELISA, ALAP Kit ELISA, APPILS Kit ELISA, ARTS1 Kit ELISA, ERAAP Kit ELISA, ERAAP1 Kit ELISA, PILS-AP Kit ELISA, PILSAP Kit ELISA, Arts1 Kit ELISA, PILSA Kit ELISA, Appils Kit ELISA, endoplasmic reticulum aminopeptidase 1 Kit ELISA, endoplasmic reticulum aminopeptidase 1b Kit ELISA, ERAP1 Kit ELISA, erap1 Kit ELISA, erap1b Kit ELISA, Erap1 Kit ELISA
    UniProt
    Q9EQH2
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