METAP2 Kit ELISA

N° du produit ABIN6730395

Détail du produit METAP2 Kit ELISA

Antigène: METAP2 (Methionyl Aminopeptidase 2 (METAP2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse METAP2 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse METAP2.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: METAP2 (Methionyl Aminopeptidase 2 (METAP2))

Autre désignation: METAP2 (METAP2 Produits)

Synonymes: metap2 Kit ELISA, MGC53792 Kit ELISA, METAP2 Kit ELISA, GB13458 Kit ELISA, DDBDRAFT_0190923 Kit ELISA, DDBDRAFT_0304991 Kit ELISA, DDB_0190923 Kit ELISA, DDB_0304991 Kit ELISA, 4930584B20Rik Kit ELISA, A930035J23Rik Kit ELISA, AI047573 Kit ELISA, AL024412 Kit ELISA, AU014659 Kit ELISA, Amp2 Kit ELISA, Mnpep Kit ELISA, p67 Kit ELISA, p67eIF2 Kit ELISA, MAP2 Kit ELISA, MNPEP Kit ELISA, wu:fb98h06 Kit ELISA, zgc:66250 Kit ELISA, methionyl aminopeptidase 2 L homeolog Kit ELISA, methionyl aminopeptidase 2 Kit ELISA, methionine aminopeptidase 2 Kit ELISA, methionine aminopeptidase Kit ELISA, methionyl aminopeptidase 2 S homeolog Kit ELISA, methionyl aminopeptidase 2b Kit ELISA, metap2.L Kit ELISA, METAP2 Kit ELISA, metap2 Kit ELISA, LOC551771 Kit ELISA, CAALFM_C604080WA Kit ELISA, Metap2 Kit ELISA, metap2.S Kit ELISA, metap2b Kit ELISA

UniProt: O08663

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling