CD73 Kit ELISA

N° du produit ABIN6730402

Détail du produit CD73 Kit ELISA

Antigène: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 7 pg/mL - 1500 pg/mL

Seuil minimal de détection: 7 pg/mL

Application: ELISA

Fonction: Mouse 5'-Nucleotidase/CD73 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse 5'-Nucleotidase.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Préparation des réactifs:

Recommended Dilution for serum and plasma samples100 fold

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Autre désignation: 5'-Nucleotidase (CD73 (NT5E Produits)

Synonymes: CALJA Kit ELISA, CD73 Kit ELISA, E5NT Kit ELISA, NT Kit ELISA, NT5 Kit ELISA, NTE Kit ELISA, eN Kit ELISA, eNT Kit ELISA, zgc:63784 Kit ELISA, NT5E Kit ELISA, MGC114869 Kit ELISA, PSPTO2277 Kit ELISA, 2210401F01Rik Kit ELISA, AI447961 Kit ELISA, Nt5 Kit ELISA, 5'-nucleotidase ecto Kit ELISA, 5'-nucleotidase, ecto (CD73) Kit ELISA, 5'-nucleotidase Kit ELISA, 5' nucleotidase, ecto Kit ELISA, NT5E Kit ELISA, nt5e Kit ELISA, nt5e.S Kit ELISA, PSPTO_2277 Kit ELISA, CBU_1233 Kit ELISA, MCA1322 Kit ELISA, Nt5e Kit ELISA

UniProt: Q61503

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process