CDK1 Kit ELISA

N° du produit ABIN6730452

Détail du produit CDK1 Kit ELISA

Antigène: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Épitope: pThr161

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Phospho-CDK1 (Thr161) ELISA Kit. This assay semi-quantitatively measures CDK1 phosphorylated at Threonine-161 in cell lysate samples.

Type d'échantillon: Cell Samples, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificité: This ELISA kit recognizes Human CDK1 phosphorylated at site Threonine-161.

Attributs du produit:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• HRP-Conjugated Secondary Antibody
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• HRP-Conjugated Secondary Antibody
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Autre désignation: CDK1 (CDK1 Produits)

Synonymes: CDC2 Kit ELISA, CDC28A Kit ELISA, P34CDC2 Kit ELISA, Cdc2 Kit ELISA, Cdc2a Kit ELISA, p34 Kit ELISA, cdc2 Kit ELISA, wu:fc30e01 Kit ELISA, zgc:92032 Kit ELISA, PSTAIR Kit ELISA, cdc-2 Kit ELISA, cdc2-a Kit ELISA, cdc28a Kit ELISA, cdc2x1.1 Kit ELISA, p34cdc2 Kit ELISA, xcdc2 Kit ELISA, 5363 Kit ELISA, CDCDm Kit ELISA, CDK1 Kit ELISA, CDK1/CDC2 Kit ELISA, CG5363 Kit ELISA, Cdk-1 Kit ELISA, Cdk1 Kit ELISA, Dcdc2 Kit ELISA, Dm cdc2 Kit ELISA, DmCdc2 Kit ELISA, DmCdk1 Kit ELISA, Dmcdc2 Kit ELISA, Dmel\\CG5363 Kit ELISA, cdc Kit ELISA, cdc2Dm Kit ELISA, cdk1 Kit ELISA, dCdk1 Kit ELISA, group 4 Kit ELISA, l(2)31Eh Kit ELISA, CDC2A Kit ELISA, CDC2AAT Kit ELISA, CDK2 Kit ELISA, CDKA1 Kit ELISA, CDKA;1 Kit ELISA, CYCLIN-DEPENDENT KINASE A;1 Kit ELISA, cell division control 2 Kit ELISA, cdc2-b Kit ELISA, cdc2a Kit ELISA, cdc2x1.2 Kit ELISA, POL3 Kit ELISA, pold Kit ELISA, cyclin dependent kinase 1 Kit ELISA, cyclin dependent kinase like 1 Kit ELISA, Cell division control protein 2 1 Kit ELISA, cyclin-dependent kinase 1 Kit ELISA, cyclin-dependent kinase 1 S homeolog Kit ELISA, Cyclin-dependent kinase 1 Kit ELISA, cell division control 2 Kit ELISA, cell division control protein2 homolog Kit ELISA, cyclin-dependent kinase 1 L homeolog Kit ELISA, polymerase (DNA directed), delta 1, catalytic subunit L homeolog Kit ELISA, cyclin-dependent protein kinase Cdk1/Cdc2 Kit ELISA, CDK1 Kit ELISA, CDKL1 Kit ELISA, POPTR_0004s14080g Kit ELISA, Cdk1 Kit ELISA, cdk1 Kit ELISA, cdk1.S Kit ELISA, CDC2 Kit ELISA, cdc2 Kit ELISA, cdk-1 Kit ELISA, cdk1.L Kit ELISA, pold1.L Kit ELISA

ID gène: 983

UniProt: P06493

Pathways: Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA