Cofilin Kit ELISA

N° du produit ABIN6730455

Détail du produit Cofilin Kit ELISA

Antigène: Cofilin (CFL1) (Cofilin 1 (CFL1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human CFL1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human CFL1.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Cofilin (CFL1) (Cofilin 1 (CFL1))

Autre désignation: CFL1 (CFL1 Produits)

Synonymes: cfl1 Kit ELISA, xac1 Kit ELISA, xac2 Kit ELISA, cfl Kit ELISA, cb86 Kit ELISA, sb:cb86 Kit ELISA, zgc:56501 Kit ELISA, wu:fb18a04 Kit ELISA, wu:fk77b03 Kit ELISA, 12/8 Kit ELISA, ADF Kit ELISA, ADF/cofilin Kit ELISA, Adf Kit ELISA, CG4254 Kit ELISA, Cadf Kit ELISA, D-cof Kit ELISA, D61 Kit ELISA, Dmel\\CG4254 Kit ELISA, T8 Kit ELISA, Tsr Kit ELISA, cofilin Kit ELISA, cofilin/ADF Kit ELISA, cofilin/actin depolymerizing factor homolog Kit ELISA, l(2)03101 Kit ELISA, l(2)k03101 Kit ELISA, l(2)k05633 Kit ELISA, l(2)k13409 Kit ELISA, ntf Kit ELISA, CFL Kit ELISA, AA959946 Kit ELISA, Cof Kit ELISA, CFL1 Kit ELISA, cofilin 1 (non-muscle) L homeolog Kit ELISA, cofilin 1 (non-muscle) Kit ELISA, cofilin 1 (non-muscle), like Kit ELISA, cofilin 1 Kit ELISA, twinstar Kit ELISA, cofilin Kit ELISA, cofilin 1, non-muscle Kit ELISA, Contrafreeloading Kit ELISA, cfl1.L Kit ELISA, cfl1 Kit ELISA, cfl1l Kit ELISA, CFL1 Kit ELISA, tsr Kit ELISA, COF1 Kit ELISA, TSTA_042790 Kit ELISA, Cfl1 Kit ELISA, CFL Kit ELISA

UniProt: P23528

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events