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DCLK1 Kit ELISA

DCLK1 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730475
  • Antigène Voir toutes DCLK1 Kits ELISA
    DCLK1 (Doublecortin-Like Kinase 1 (DCLK1))
    Reactivité
    • 4
    • 2
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human DCLK1 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Human DCLK1.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product DCLK1 Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes DCLK1 Kits ELISA
    DCLK1 (Doublecortin-Like Kinase 1 (DCLK1))
    Autre désignation
    DCLK1 (DCLK1 Produits)
    Synonymes
    DCLK1 Kit ELISA, DCAMKL1 Kit ELISA, dcamkl1 Kit ELISA, dclk1 Kit ELISA, wu:fc51f11 Kit ELISA, zgc:153709 Kit ELISA, MGC145348 Kit ELISA, CL1 Kit ELISA, CLICK1 Kit ELISA, DCDC3A Kit ELISA, DCLK Kit ELISA, 1700113D08Rik Kit ELISA, 2810480F11Rik Kit ELISA, AI836758 Kit ELISA, Click-I Kit ELISA, Cpg16 Kit ELISA, Dcamkl1 Kit ELISA, Dcl Kit ELISA, Dclk Kit ELISA, mKIAA0369 Kit ELISA, Ania4 Kit ELISA, doublecortin like kinase 1 Kit ELISA, doublecortin-like kinase 1a Kit ELISA, serine/threonine-protein kinase DCLK1 Kit ELISA, doublecortin-like kinase 2 Kit ELISA, doublecortin-like kinase 1 Kit ELISA, DCLK1 Kit ELISA, dclk1a Kit ELISA, LOC587664 Kit ELISA, dclk2 Kit ELISA, LOC100539645 Kit ELISA, Dclk1 Kit ELISA
    UniProt
    O15075
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