DDR2 Kit ELISA

N° du produit ABIN6730477

Détail du produit DDR2 Kit ELISA

Antigène: DDR2 (Discoidin Domain Receptor tyrosine Kinase 2 (DDR2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse DDR2 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse DDR2.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: DDR2 (Discoidin Domain Receptor tyrosine Kinase 2 (DDR2))

Autre désignation: DDR2 (DDR2 Produits)

Synonymes: MIG20a Kit ELISA, NTRKR3 Kit ELISA, TKT Kit ELISA, TYRO10 Kit ELISA, AW495251 Kit ELISA, Ntrkr3 Kit ELISA, tyro10 Kit ELISA, DDR2 Kit ELISA, Tyro10 Kit ELISA, ddr2 Kit ELISA, si:ch211-193c2.1 Kit ELISA, discoidin domain receptor tyrosine kinase 2 Kit ELISA, discoidin domain receptor family, member 2 Kit ELISA, discoidin domain receptor family, member 2, like Kit ELISA, DDR2 Kit ELISA, Ddr2 Kit ELISA, ddr2l Kit ELISA

UniProt: Q62371

Pathways: Signalisation RTK