GNS Kit ELISA

N° du produit ABIN6730488

Détail du produit GNS Kit ELISA

Antigène: GNS (Glucosamine (N-Acetyl)-6-Sulfatase (GNS))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Glucosamine/N-acetyl-6-Sulfatase ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human Glucosamine.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: GNS (Glucosamine (N-Acetyl)-6-Sulfatase (GNS))

Autre désignation: Glucosamine (N-acetyl-6-Sulfatase (GNS Produits)

Synonymes: G6S Kit ELISA, 2610016K11Rik Kit ELISA, AU042285 Kit ELISA, C87209 Kit ELISA, N28088 Kit ELISA, NV14559 Kit ELISA, N-acetylglucosamine-6-sulfatase Kit ELISA, zgc:114066 Kit ELISA, gns Kit ELISA, wu:fi20h10 Kit ELISA, zgc:55370 Kit ELISA, glucosamine (N-acetyl)-6-sulfatase Kit ELISA, glucosamine (N-acetyl)-6-sulfatase S homeolog Kit ELISA, glucosamine (N-acetyl)-6-sulfatase a Kit ELISA, N-acetylglucosamine-6-sulfatase Kit ELISA, glucosamine (N-acetyl)-6-sulfatase (Sanfilippo disease IIID), b Kit ELISA, GNS Kit ELISA, Gns Kit ELISA, gns.S Kit ELISA, gns Kit ELISA, gnsa Kit ELISA, CpipJ_CPIJ000745 Kit ELISA, Sros_7372 Kit ELISA, VDBG_04409 Kit ELISA, Halhy_3165 Kit ELISA, gnsb Kit ELISA

UniProt: P15586

Pathways: Glycosaminoglycan Metabolic Process