×
Pour une meilleure utilisation, nous vous conseillons d’activer Javascript dans votre navigateur.
MEK1 Kit ELISA
MAP2K1
Reactivité: Souris
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730500
Détail du produit MEK1 Kit ELISA
(cache)
Antigène
Voir toutes MEK1 (MAP2K1) Kits ELISA
MEK1 (MAP2K1)
(Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
Reactivité
Toutes les réactivités sur MEK1 Kits ELISA
Souris
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Mouse MAP2K1 ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes Mouse MAP2K1.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Top Product
Discover our top product MAP2K1 Kit ELISA
Alternatives
(show)
Information d'application
(cache)
Indications d'application
Optimal working dilution should be determined by the investigator.
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
(cache)
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
(cache)
Antigène
Voir toutes MEK1 (MAP2K1) Kits ELISA
MEK1 (MAP2K1)
(Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
Autre désignation
MAP2K1 (MAP2K1 Produits )
Synonymes
Mek1 Kit ELISA, CFC3 Kit ELISA, MAPKK1 Kit ELISA, MEK1 Kit ELISA, MKK1 Kit ELISA, PRKMK1 Kit ELISA, MEKK1 Kit ELISA, Prkmk1 Kit ELISA, mek-2 Kit ELISA, si:ch211-242m18.2 Kit ELISA, wu:fj56a12 Kit ELISA, wu:fj61b01 Kit ELISA, zgc:56557 Kit ELISA, ATMEK1 Kit ELISA, F20B18.180 Kit ELISA, F20B18_180 Kit ELISA, MAP kinase/ ERK kinase 1 Kit ELISA, MITOGEN ACTIVATED PROTEIN KINASE KINASE Kit ELISA, MITOGEN ACTIVATED PROTEIN KINASE KINASE 1 Kit ELISA, NMAPKK Kit ELISA, MEK Kit ELISA, mitogen activated protein kinase kinase 1 Kit ELISA, mitogen-activated protein kinase kinase 1 Kit ELISA, mitogen-activated protein kinase kinase 1 L homeolog Kit ELISA, MAP kinase/ ERK kinase 1 Kit ELISA, Map2k1 Kit ELISA, MAP2K1 Kit ELISA, map2k1.L Kit ELISA, map2k1 Kit ELISA, MEK1 Kit ELISA
UniProt
P31938
Pathways
Signalisation MAPK , Signalisation RTK , Interferon-gamma Pathway , Fc-epsilon Receptor Signaling Pathway , Neurotrophin Signaling Pathway , Activation of Innate immune Response , Toll-Like Receptors Cascades , Autophagy , Signaling of Hepatocyte Growth Factor Receptor , BCR Signaling
Vus récemment
(cache)