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TGFBR2 Kit ELISA
TGFBR2
Reactivité: Souris
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730518
Détail du produit TGFBR2 Kit ELISA
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Antigène
Voir toutes TGFBR2 Kits ELISA
TGFBR2
(Transforming Growth Factor, beta Receptor II (70/80kDa) (TGFBR2))
Reactivité
Toutes les réactivités sur TGFBR2 Kits ELISA
Souris
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Mouse TGF-beta RII ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes Mouse TGF-beta RII.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Alternatives
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Information d'application
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Indications d'application
Optimal working dilution should be determined by the investigator.
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes TGFBR2 Kits ELISA
TGFBR2
(Transforming Growth Factor, beta Receptor II (70/80kDa) (TGFBR2))
Autre désignation
TGF-beta RII (TGFBR2 Produits )
Synonymes
AAT3 Kit ELISA, FAA3 Kit ELISA, LDS1B Kit ELISA, LDS2B Kit ELISA, MFS2 Kit ELISA, RIIC Kit ELISA, TAAD2 Kit ELISA, TGFR-2 Kit ELISA, TGFbeta-RII Kit ELISA, 1110020H15Rik Kit ELISA, AU042018 Kit ELISA, DNIIR Kit ELISA, RIIDN Kit ELISA, TBR-II Kit ELISA, TbetaR-II Kit ELISA, TbetaRII Kit ELISA, TGFBR2 Kit ELISA, TBETA-RII Kit ELISA, TGFBRII Kit ELISA, TGF-beta 2 Kit ELISA, Tgfbr2T Kit ELISA, cb537 Kit ELISA, tgfbr2 Kit ELISA, wu:fj05c10 Kit ELISA, zgc:110498 Kit ELISA, transforming growth factor beta receptor 2 Kit ELISA, transforming growth factor, beta receptor II Kit ELISA, transforming growth factor, beta receptor 2 Kit ELISA, transforming growth factor beta receptor 2b Kit ELISA, TGFBR2 Kit ELISA, Tgfbr2 Kit ELISA, tgfbr2b Kit ELISA
UniProt
Q62312
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