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PRKAR1A Kit ELISA
PRKAR1A
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730540
Détail du produit PRKAR1A Kit ELISA
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Antigène
Tous les produits PRKAR1A
PRKAR1A
(Protein Kinase, CAMP-Dependent, Regulatory, Type I, alpha (Tissue Specific Extinguisher 1) (PRKAR1A))
Reactivité
Toutes les réactivités sur PRKAR1A Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Human PRKAR1A ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes Human PRKAR1A.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternatives
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Information d'application
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Indications d'application
Optimal working dilution should be determined by the investigator.
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Tous les produits PRKAR1A
PRKAR1A
(Protein Kinase, CAMP-Dependent, Regulatory, Type I, alpha (Tissue Specific Extinguisher 1) (PRKAR1A))
Autre désignation
PRKAR1A (PRKAR1A Produits )
Synonymes
ACRDYS1 Kit ELISA, ADOHR Kit ELISA, CAR Kit ELISA, CNC Kit ELISA, CNC1 Kit ELISA, PKR1 Kit ELISA, PPNAD1 Kit ELISA, PRKAR1 Kit ELISA, TSE1 Kit ELISA, 1300018C22Rik Kit ELISA, RIalpha Kit ELISA, Tse-1 Kit ELISA, Tse1 Kit ELISA, RIIA Kit ELISA, prkar1a Kit ELISA, zgc:92515 Kit ELISA, im:7047729 Kit ELISA, cnc1 Kit ELISA, pkr1 Kit ELISA, ppnad1 Kit ELISA, prkar1 Kit ELISA, tse1 Kit ELISA, protein kinase cAMP-dependent type I regulatory subunit alpha Kit ELISA, protein kinase, cAMP dependent regulatory, type I, alpha Kit ELISA, protein kinase cAMP-dependent type 1 regulatory subunit alpha Kit ELISA, protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1) a Kit ELISA, cAMP-dependent protein kinase type I-alpha regulatory subunit pseudogene Kit ELISA, protein kinase cAMP-dependent type I regulatory subunit alpha S homeolog Kit ELISA, PRKAR1A Kit ELISA, Prkar1a Kit ELISA, prkar1aa Kit ELISA, LOC707275 Kit ELISA, prkar1a.S Kit ELISA
UniProt
P10644
Pathways
Signalisation Hedgehog , EGFR Signaling Pathway , Neurotrophin Signaling Pathway , Myometrial Relaxation and Contraction , G-protein mediated Events , Interaction of EGFR with phospholipase C-gamma
Vus récemment
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