YWHAE Kit ELISA

N° du produit ABIN6730561

Détail du produit YWHAE Kit ELISA

Antigène: YWHAE (14-3-3 epsilon (YWHAE))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.34 ng/mL - 80 ng/mL

Seuil minimal de détection: 0.34 ng/mL

Application: ELISA

Fonction: Human YWHAE ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human YWHAE.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Préparation des réactifs:

Recommended Dilution for serum and plasma samples2 fold

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: YWHAE (14-3-3 epsilon (YWHAE))

Autre désignation: YWHAE (YWHAE Produits)

Synonymes: 14-3-3 Kit ELISA, 14-3-3 epsilon Kit ELISA, 14-3-3-e Kit ELISA, 14-3-3-epsilon Kit ELISA, 14-3-30epsilon Kit ELISA, 14-3-3EPSILON Kit ELISA, 14-3-3e Kit ELISA, 14-3-3omicron Kit ELISA, 1433epsilon Kit ELISA, 143E_DROME Kit ELISA, CG31196 Kit ELISA, CT24092 Kit ELISA, D14-3-3e Kit ELISA, D14-3-3epsilon Kit ELISA, Dmel\\CG31196 Kit ELISA, EK3-5 Kit ELISA, PAR-5 Kit ELISA, PAR5 Kit ELISA, Par-5 Kit ELISA, SR3-9 Kit ELISA, Su(Raf)3B Kit ELISA, anon-WO0172774.141 Kit ELISA, anon-WO02059370.52 Kit ELISA, d14-3-3epsilon Kit ELISA, eps Kit ELISA, l(3)j2B10 Kit ELISA, par-5 Kit ELISA, 14-3-3epsilon Kit ELISA, kcip-1 Kit ELISA, mdcr Kit ELISA, mds Kit ELISA, LOC581376 Kit ELISA, Ywhae Kit ELISA, ACYPI002517 Kit ELISA, YWHAE Kit ELISA, 14-3-3E Kit ELISA, KCIP-1 Kit ELISA, MDCR Kit ELISA, MDS Kit ELISA, AU019196 Kit ELISA, fe38c12 Kit ELISA, wu:fb48f06 Kit ELISA, wu:fe38c12 Kit ELISA, wu:fi22e10 Kit ELISA, ywhae Kit ELISA, zgc:55377 Kit ELISA, zgc:77062 Kit ELISA, CG31196 gene product from transcript CG31196-RD Kit ELISA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon Kit ELISA, 14-3-3 epsilon Kit ELISA, 14-3-3 epsilon protein Kit ELISA, 14-3-3 protein epsilon Kit ELISA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide Kit ELISA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon Kit ELISA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon S homeolog Kit ELISA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide 1 Kit ELISA, 14-3-3epsilon Kit ELISA, ywhae Kit ELISA, LOC581376 Kit ELISA, Ywhae Kit ELISA, HCAG_04173 Kit ELISA, PTRG_02836 Kit ELISA, PTRG_04459 Kit ELISA, UREG_07864 Kit ELISA, YWHAE Kit ELISA, ywhae.S Kit ELISA, ywhae1 Kit ELISA

UniProt: P62258

Pathways: Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, M Phase