SEMA4D/CD100 Kit ELISA
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- Antigène Voir toutes SEMA4D/CD100 (SEMA4D) Kits ELISA
- SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 23 pg/mL - 5000 pg/mL
- Seuil minimal de détection
- 23 pg/mL
- Application
- ELISA
- Fonction
- Mouse Semaphorin 4D/CD100 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
- Type d'échantillon
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- Specificité
- This ELISA antibody pair recognizes Mouse Semaphorin 4D.
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product SEMA4D Kit ELISA
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- Préparation des réactifs
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Recommended Dilution for serum and plasma samples15 fold
- Restrictions
- For Research Use only
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- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
-
- Antigène Voir toutes SEMA4D/CD100 (SEMA4D) Kits ELISA
- SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))
- Autre désignation
- Semaphorin 4D (CD100 (SEMA4D Produits)
- Synonymes
- C9orf164 Kit ELISA, CD100 Kit ELISA, M-sema-G Kit ELISA, SEMAJ Kit ELISA, coll-4 Kit ELISA, Semacl2 Kit ELISA, Semaj Kit ELISA, Semcl2 Kit ELISA, COLL-4 Kit ELISA, semaphorin 4D Kit ELISA, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D Kit ELISA, Sema4d Kit ELISA, SEMA4D Kit ELISA
- UniProt
- O09126
- Pathways
- Regulation of Cell Size
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