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SERPINA10 Kit ELISA
SERPINA10
Reactivité: Souris
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730611
Détail du produit SERPINA10 Kit ELISA
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Antigène
Voir toutes SERPINA10 Kits ELISA
SERPINA10
(serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 10 (SERPINA10))
Reactivité
Toutes les réactivités sur SERPINA10 Kits ELISA
Souris
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Mouse Serpina10 ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes Mouse Serpina10.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Indications d'application
Optimal working dilution should be determined by the investigator.
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes SERPINA10 Kits ELISA
SERPINA10
(serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 10 (SERPINA10))
Autre désignation
Serpina10 (SERPINA10 Produits )
Synonymes
PZI Kit ELISA, ZPI Kit ELISA, Rasp1 Kit ELISA, SERPINA10 Kit ELISA, DKFZp469K045 Kit ELISA, si:dkey-85n7.5 Kit ELISA, serpin family A member 10 Kit ELISA, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10 Kit ELISA, serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10 Kit ELISA, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10a Kit ELISA, SERPINA10 Kit ELISA, Serpina10 Kit ELISA, serpina10a Kit ELISA
UniProt
Q8R121
Vus récemment
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