KIAA0101 Kit ELISA

N° du produit ABIN6730630

Détail du produit KIAA0101 Kit ELISA

Antigène: KIAA0101 (PAF) (PCNA Clamp Associated Factor (PAF))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human KIAA0101/PCLAF ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human KIAA0101.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: KIAA0101 (PAF) (PCNA Clamp Associated Factor (PAF))

Autre désignation: KIAA0101 (PCLAF (PAF Produits)

Synonymes: NS5ATP9 Kit ELISA, OEATC Kit ELISA, OEATC-1 Kit ELISA, OEATC1 Kit ELISA, PAF Kit ELISA, PAF15 Kit ELISA, p15(PAF) Kit ELISA, p15/PAF Kit ELISA, p15PAF Kit ELISA, paf Kit ELISA, Paf Kit ELISA, AA409629 Kit ELISA, Ns5apt9 Kit ELISA, Ns5atp9 Kit ELISA, mKIAA0101 Kit ELISA, Kiaa0101 Kit ELISA, PCNA clamp associated factor Kit ELISA, PCNA clamp associated factor S homeolog Kit ELISA, PCLAF Kit ELISA, pclaf Kit ELISA, pclaf.S Kit ELISA, Pclaf Kit ELISA

UniProt: Q15004

Pathways: Chromatin Binding