GLA Kit ELISA

N° du produit ABIN6730640

Détail du produit GLA Kit ELISA

Antigène: GLA (Galactosidase, alpha (GLA))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse GLA ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse GLA.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: GLA (Galactosidase, alpha (GLA))

Autre désignation: GLA (GLA Produits)

Synonymes: GALA Kit ELISA, Ags Kit ELISA, zgc:101584 Kit ELISA, MGC130872 Kit ELISA, SMU.877 Kit ELISA, SCF11.21 Kit ELISA, AO090005000217 Kit ELISA, alpha-GAL Kit ELISA, galactosidase alpha Kit ELISA, galactosidase, alpha Kit ELISA, galactosidase alpha S homeolog Kit ELISA, alpha-galactosidase Kit ELISA, aga Kit ELISA, alpha-galactosidase A Kit ELISA, GLA Kit ELISA, Gla Kit ELISA, gla Kit ELISA, gla.S Kit ELISA, agaN Kit ELISA, aga Kit ELISA, agaL Kit ELISA, SCO0541 Kit ELISA, rafA Kit ELISA, melA Kit ELISA, galA Kit ELISA, ANI_1_2528074 Kit ELISA, ANI_1_1502124 Kit ELISA, AOR_1_390174 Kit ELISA, CpipJ_CPIJ002066 Kit ELISA, MCYG_00962 Kit ELISA, MCYG_00791 Kit ELISA, Tsp_02909 Kit ELISA, Tsp_02508 Kit ELISA

UniProt: Q8BGZ6

Pathways: SARS-CoV-2 Protein Interactome