CHI3L1 Kit ELISA

N° du produit ABIN6730660

Détail du produit CHI3L1 Kit ELISA

Antigène: CHI3L1 (Chitinase 3-Like 1 (Cartilage Glycoprotein-39) (CHI3L1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse Chitinase 3-like 1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse Chitinase 3-like 1.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: CHI3L1 (Chitinase 3-Like 1 (Cartilage Glycoprotein-39) (CHI3L1))

Autre désignation: Chitinase 3-like 1 (CHI3L1 Produits)

Synonymes: AW208766 Kit ELISA, Brp39 Kit ELISA, Gp39 Kit ELISA, ASRT7 Kit ELISA, CGP-39 Kit ELISA, GP-39 Kit ELISA, GP39 Kit ELISA, HC-gp39 Kit ELISA, HCGP-3P Kit ELISA, YKL-40 Kit ELISA, YKL40 Kit ELISA, YYL-40 Kit ELISA, hCGP-39 Kit ELISA, GP38K Kit ELISA, CLP-1 Kit ELISA, SPC-40 Kit ELISA, SPS-40 Kit ELISA, Chil1 Kit ELISA, BP40 Kit ELISA, MGP-40 Kit ELISA, chitinase-like 1 Kit ELISA, chitinase 3 like 1 Kit ELISA, chitinase 3-like 1 (cartilage glycoprotein-39) Kit ELISA, Chil1 Kit ELISA, CHI3L1 Kit ELISA, Chi3l1 Kit ELISA

Classe de substances: Viral Protein

UniProt: Q61362