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FZD2 Kit ELISA
FZD2
Reactivité: Souris
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730675
Détail du produit FZD2 Kit ELISA
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Antigène
Tous les produits FZD2
FZD2
(Frizzled Family Receptor 2 (FZD2))
Reactivité
Souris
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Mouse Frizzled-2 ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes Mouse Frizzled-2.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternatives
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Information d'application
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Indications d'application
Optimal working dilution should be determined by the investigator.
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Tous les produits FZD2
FZD2
(Frizzled Family Receptor 2 (FZD2))
Autre désignation
Frizzled-2 (FZD2 Produits )
Synonymes
BG01711 Kit ELISA, CG9739 Kit ELISA, D-Fz2 Kit ELISA, D-fz2 Kit ELISA, DFZ2 Kit ELISA, DFz-2 Kit ELISA, DFz2 Kit ELISA, Dfrz2 Kit ELISA, Dfz2 Kit ELISA, Dfz[[2]] Kit ELISA, Dm Fz2 Kit ELISA, Dmel\\CG9739 Kit ELISA, FZ2 Kit ELISA, Fz2 Kit ELISA, dFz2 Kit ELISA, dfz(2) Kit ELISA, dfz2 Kit ELISA, dfz2r Kit ELISA, frz2 Kit ELISA, fz-2 Kit ELISA, fz8 Kit ELISA, zfz2 Kit ELISA, zg08 Kit ELISA, cb383 Kit ELISA, wu:fb70g09 Kit ELISA, FZD2 Kit ELISA, fzE2 Kit ELISA, hFz2 Kit ELISA, Fz-10 Kit ELISA, Fzd10 Kit ELISA, cFz-2 Kit ELISA, frizzled2 Kit ELISA, fz2 Kit ELISA, xfz2 Kit ELISA, AL033370 Kit ELISA, AW456835 Kit ELISA, Fz10 Kit ELISA, Mfz10 Kit ELISA, Mfz10a Kit ELISA, frizzled 2 Kit ELISA, frizzled class receptor 2 Kit ELISA, frizzled class receptor 2 S homeolog Kit ELISA, fz2 Kit ELISA, fzd2 Kit ELISA, FZD2 Kit ELISA, Fzd2 Kit ELISA, fzd2.S Kit ELISA
UniProt
Q9JIP6
Pathways
Signalisation WNT
Vus récemment
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