HSC70 Interacting Protein HIP Kit ELISA

N° du produit ABIN6730680

Détail du produit HSC70 Interacting Protein HIP Kit ELISA

Antigène: HSC70 Interacting Protein HIP (ST13) (Suppression of Tumorigenicity 13 (ST13))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse Hip ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse Hip.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: HSC70 Interacting Protein HIP (ST13) (Suppression of Tumorigenicity 13 (ST13))

Autre désignation: Hip (ST13 Produits)

Synonymes: CG32789 Kit ELISA, Dmel\\CG32789 Kit ELISA, anon-EST:fe1A9 Kit ELISA, 1110007I03Rik Kit ELISA, 3110002K08Rik Kit ELISA, AW555194 Kit ELISA, HIP Kit ELISA, HOP Kit ELISA, HSPABP Kit ELISA, HSPABP1 Kit ELISA, PRO0786 Kit ELISA, SNC6 Kit ELISA, p48 Kit ELISA, AAG2 Kit ELISA, FAM10A1 Kit ELISA, FAM10A4 Kit ELISA, P48 Kit ELISA, Hsc/Hsp70-interacting protein Kit ELISA, suppression of tumorigenicity 13 Kit ELISA, ST13, Hsp70 interacting protein Kit ELISA, suppression of tumorigenicity 13 (colon carcinoma) (Hsp70 interacting protein) Kit ELISA, HIP Kit ELISA, St13 Kit ELISA, ST13 Kit ELISA

UniProt: Q7TN16