LMAN2 Kit ELISA

N° du produit ABIN6730685

Détail du produit LMAN2 Kit ELISA

Antigène: LMAN2 (Lectin, Mannose-Binding 2 (LMAN2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human LMAN2 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human LMAN2.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: LMAN2 (Lectin, Mannose-Binding 2 (LMAN2))

Autre désignation: LMAN2 (LMAN2 Produits)

Synonymes: Lman2 Kit ELISA, wu:fb16f04 Kit ELISA, zgc:158761 Kit ELISA, C5orf8 Kit ELISA, GP36B Kit ELISA, VIP36 Kit ELISA, 1110003H06Rik Kit ELISA, 1300009F09Rik Kit ELISA, AA408240 Kit ELISA, AL023023 Kit ELISA, AU040819 Kit ELISA, Vip36 Kit ELISA, lectin, mannose binding 2 Kit ELISA, lectin, mannose-binding 2 Kit ELISA, LMAN2 Kit ELISA, lman2 Kit ELISA, Lman2 Kit ELISA

UniProt: Q12907

Pathways: SARS-CoV-2 Protein Interactome