SPARC Kit ELISA

N° du produit ABIN6730690

Détail du produit SPARC Kit ELISA

Antigène: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse SPARC ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse SPARC.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Autre désignation: SPARC (SPARC Produits)

Synonymes: ON Kit ELISA, AA517111 Kit ELISA, BM-40 Kit ELISA, hm:zeh0062 Kit ELISA, SPARC Kit ELISA, sparc Kit ELISA, secreted protein acidic and cysteine rich Kit ELISA, secreted acidic cysteine rich glycoprotein Kit ELISA, secreted protein, acidic, cysteine-rich (osteonectin) Kit ELISA, secreted protein, acidic, cysteine-rich (osteonectin) L homeolog Kit ELISA, secreted protein, acidic, cysteine-rich (osteonectin) S homeolog Kit ELISA, SPARC Kit ELISA, Sparc Kit ELISA, sparc Kit ELISA, sparc.L Kit ELISA, sparc.S Kit ELISA

UniProt: P07214

Pathways: Autophagy