GPHa2 Kit ELISA

N° du produit ABIN6730699

Détail du produit GPHa2 Kit ELISA

Antigène: GPHa2 (Glycoprotein Hormone alpha 2 (GPHa2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human GPHA2 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human GPHA2.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: GPHa2 (Glycoprotein Hormone alpha 2 (GPHa2))

Autre désignation: GPHA2 (GPHa2 Produits)

Synonymes: GLEAN_11941 Kit ELISA, CG17878 Kit ELISA, Dmel\\CG17878 Kit ELISA, GPA2 Kit ELISA, Gpa Kit ELISA, gpa2 Kit ELISA, A2 Kit ELISA, ZSIG51 Kit ELISA, zsig51 Kit ELISA, Zsig51 Kit ELISA, glycoprotein hormone alpha 2 Kit ELISA, Glycoprotein hormone alpha 2 Kit ELISA, GPHA2 Kit ELISA, Gpa2 Kit ELISA, CpipJ_CPIJ013236 Kit ELISA, GPA2 Kit ELISA, gpha2 Kit ELISA, Gpha2 Kit ELISA

UniProt: Q96T91

Pathways: Hormone Activity