Cholesterol Esterase Kit ELISA

N° du produit ABIN6730707

Détail du produit Cholesterol Esterase Kit ELISA

Antigène: Cholesterol Esterase (CEL)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse CEL ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse CEL.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Cholesterol Esterase (CEL)

Autre désignation: CEL (CEL Produits)

Synonymes: SC8F4.24 Kit ELISA, BAL Kit ELISA, BSDL Kit ELISA, BSSL Kit ELISA, CELL Kit ELISA, CEase Kit ELISA, FAP Kit ELISA, FAPP Kit ELISA, LIPA Kit ELISA, MODY8 Kit ELISA, 1810036E18Rik Kit ELISA, Bal Kit ELISA, Bssl Kit ELISA, carboxyl ester lipase Kit ELISA, cholesterol esterase Kit ELISA, hypothetical protein Kit ELISA, CEL Kit ELISA, SCO5420 Kit ELISA, Tfu_2331 Kit ELISA, SARE_RS01070 Kit ELISA, RSal33209_1252 Kit ELISA, Caci_5976 Kit ELISA, Ndas_0054 Kit ELISA, Micau_5972 Kit ELISA, ML5_2523 Kit ELISA, Cel Kit ELISA, cel Kit ELISA

UniProt: Q64285

Pathways: Lipid Metabolism