Prothrombin Kit ELISA

N° du produit ABIN6730721

Détail du produit Prothrombin Kit ELISA

Antigène: Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse Coagulation Factor II/F2 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse Coagulation Factor II.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Autre désignation: Coagulation Factor II (F2 (F2 Produits)

Synonymes: PT Kit ELISA, RPRGL2 Kit ELISA, THPH1 Kit ELISA, Cf-2 Kit ELISA, Cf2 Kit ELISA, FII Kit ELISA, thrombin Kit ELISA, zgc:66319 Kit ELISA, wu:fb57c10 Kit ELISA, wu:fd42e09 Kit ELISA, wu:fd59d01 Kit ELISA, prothrombin Kit ELISA, F2 Kit ELISA, coagulation factor II, thrombin Kit ELISA, coagulation factor II Kit ELISA, coagulation factor II (thrombin) Kit ELISA, F2 Kit ELISA, f2 Kit ELISA

UniProt: P19221

Pathways: Système du Complément, Peptide Hormone Metabolism, Regulation of G-Protein Coupled Receptor Protein Signaling