CD63 Kit ELISA
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- Antigène Voir toutes CD63 Kits ELISA
- CD63
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 14 pg/mL - 3000 pg/mL
- Seuil minimal de détection
- 14 pg/mL
- Application
- ELISA
- Fonction
- Human CD63 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
- Type d'échantillon
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Quantitative
- Specificité
- This ELISA antibody pair recognizes Human CD63.
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product CD63 Kit ELISA
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- Préparation des réactifs
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Recommended Dilution for serum and plasma samples2 fold
- Restrictions
- For Research Use only
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- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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- Antigène Voir toutes CD63 Kits ELISA
- CD63
- Autre désignation
- CD63 (CD63 Produits)
- Synonymes
- C75951 Kit ELISA, ME491 Kit ELISA, Tspan30 Kit ELISA, LAMP-3 Kit ELISA, MLA1 Kit ELISA, OMA81H Kit ELISA, TSPAN30 Kit ELISA, TSP29FA Kit ELISA, CD63 antigen Kit ELISA, CD63 molecule Kit ELISA, Cd63 molecule Kit ELISA, Cd63 Kit ELISA, CD63 Kit ELISA
- UniProt
- P08962
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