Notch1 Kit ELISA

N° du produit ABIN6730753

Détail du produit Notch1 Kit ELISA

Antigène: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse Notch-1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse Notch-1.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Autre désignation: Notch-1 (NOTCH1 Produits)

Synonymes: notch1 Kit ELISA, NOTCH1 Kit ELISA, hn1 Kit ELISA, tan1 Kit ELISA, notch Kit ELISA, xotch Kit ELISA, xnotch Kit ELISA, notch-1 Kit ELISA, xnotch1 Kit ELISA, TAN1 Kit ELISA, hN1 Kit ELISA, 9930111A19Rik Kit ELISA, Mis6 Kit ELISA, N1 Kit ELISA, Tan1 Kit ELISA, lin-12 Kit ELISA, NOTCH Kit ELISA, Xotch Kit ELISA, notch1-a Kit ELISA, notch 1 Kit ELISA, fibropellin-1 Kit ELISA, Notch homolog 1, translocation-associated (Drosophila) Kit ELISA, Notch homolog 1, translocation-associated Kit ELISA, notch 1 S homeolog Kit ELISA, notch1 Kit ELISA, LOC100631526 Kit ELISA, NOTCH1 Kit ELISA, Notch1 Kit ELISA, notch1.S Kit ELISA

UniProt: Q01705

Pathways: Signalisation Notch, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development