PLA2G2A Kit ELISA

N° du produit ABIN6730778

Détail du produit PLA2G2A Kit ELISA

Antigène: PLA2G2A (Phospholipase A2, Group IIA (Platelets, Synovial Fluid) (PLA2G2A))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse PLA2G2A ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse PLA2G2A.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: PLA2G2A (Phospholipase A2, Group IIA (Platelets, Synovial Fluid) (PLA2G2A))

Autre désignation: PLA2G2A (PLA2G2A Produits)

Synonymes: MOM1 Kit ELISA, PLA2 Kit ELISA, PLA2B Kit ELISA, PLA2L Kit ELISA, PLA2S Kit ELISA, PLAS1 Kit ELISA, sPLA2 Kit ELISA, EF Kit ELISA, Mom1 Kit ELISA, Pla2 Kit ELISA, sPla2-IIA Kit ELISA, phospholipase A2 group IIA Kit ELISA, phospholipase A2, group IIA (platelets, synovial fluid) Kit ELISA, PLA2G2A Kit ELISA, Pla2g2a Kit ELISA

UniProt: P31482

Pathways: Stem Cell Maintenance, Inositol Metabolic Process