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CHRNB3 Kit ELISA
CHRNB3
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730816
Détail du produit CHRNB3 Kit ELISA
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Antigène
Voir toutes CHRNB3 Kits ELISA
CHRNB3
(Cholinergic Receptor, Nicotinic, beta 3 (Neuronal) (CHRNB3))
Reactivité
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Human CHRNB3 ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes Human CHRNB3.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Alternatives
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Information d'application
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Indications d'application
Optimal working dilution should be determined by the investigator.
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes CHRNB3 Kits ELISA
CHRNB3
(Cholinergic Receptor, Nicotinic, beta 3 (Neuronal) (CHRNB3))
Autre désignation
CHRNB3 (CHRNB3 Produits )
Synonymes
5730417K16Rik Kit ELISA, Acrb3 Kit ELISA, NACHR Kit ELISA, CHRNB3 Kit ELISA, chrnb3 Kit ELISA, cholinergic receptor nicotinic beta 3 subunit Kit ELISA, neuronal acetylcholine receptor subunit beta-3 Kit ELISA, cholinergic receptor, nicotinic, beta polypeptide 3 Kit ELISA, cholinergic receptor, nicotinic beta 3 L homeolog Kit ELISA, cholinergic receptor, nicotinic beta 3 Kit ELISA, cholinergic receptor, nicotinic, beta 3b (neuronal) Kit ELISA, cholinergic receptor, nicotinic, beta polypeptide 3a Kit ELISA, CHRNB3 Kit ELISA, CpipJ_CPIJ012638 Kit ELISA, Chrnb3 Kit ELISA, chrnb3.L Kit ELISA, chrnb3 Kit ELISA, chrnb3b Kit ELISA, chrnb3a Kit ELISA
UniProt
Q05901
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