CADM1 Kit ELISA

N° du produit ABIN6810493

Détail du produit CADM1 Kit ELISA

Antigène: CADM1 (Cell Adhesion Molecule 1 (CADM1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse IGSF4A/SynCAM1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse IGSF4A.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Date de péremption: 6 months

Détail du antigène

Antigène: CADM1 (Cell Adhesion Molecule 1 (CADM1))

Autre désignation: IGSF4A (CADM1 Produits)

Synonymes: BL2 Kit ELISA, IGSF4 Kit ELISA, IGSF4A Kit ELISA, NECL2 Kit ELISA, Necl-2 Kit ELISA, RA175 Kit ELISA, ST17 Kit ELISA, SYNCAM Kit ELISA, TSLC1 Kit ELISA, sTSLC-1 Kit ELISA, sgIGSF Kit ELISA, synCAM1 Kit ELISA, bl2 Kit ELISA, igsf4 Kit ELISA, igsf4a Kit ELISA, necl-2 Kit ELISA, necl2 Kit ELISA, ra175 Kit ELISA, sgigsf Kit ELISA, st17 Kit ELISA, stslc-1 Kit ELISA, syncam Kit ELISA, syncam1 Kit ELISA, tslc1 Kit ELISA, xnecl2 Kit ELISA, 2900073G06Rik Kit ELISA, 3100001I08Rik Kit ELISA, AI987920 Kit ELISA, Bl2 Kit ELISA, Igsf4 Kit ELISA, Igsf4a Kit ELISA, Necl2 Kit ELISA, RA175A Kit ELISA, RA175B Kit ELISA, RA175C Kit ELISA, RA175N Kit ELISA, SgIGSF Kit ELISA, SynCam Kit ELISA, Tslc1 Kit ELISA, SynCAM1 Kit ELISA, cell adhesion molecule 1 Kit ELISA, cell adhesion molecule 1 L homeolog Kit ELISA, CADM1 Kit ELISA, cadm1.L Kit ELISA, Cadm1 Kit ELISA

UniProt: Q8R5M8

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Activated T Cell Proliferation