Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

KIT Kit IQ-ELISA

KIT Reactivité: Humain qPCR DNA-Binding ELISA Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN6810500
  • Antigène Voir toutes KIT IQ-ELISA Kits
    KIT (Mast/stem Cell Growth Factor Receptor (KIT))
    Reactivité
    • 11
    • 10
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    qPCR
    Type de méthode
    DNA-Binding ELISA
    Application
    ELISA
    Fonction
    Human Immunoquantitative (PCR-Based) SCF R (CD117/c-kit) ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Type d'échantillon
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Sensibilité
    4.5 pg/mL
    Attributs du produit
    The Immuno Quantitative Enzyme Linked ImumunoSorbent Assay (IQELISA™) is an innovative new assay that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of lower sample volumes while also providing more sensitivity. The Human SCF R (CD117/c-kit) IQELISA™ Kit is a modified ELISA assay with high sensitivity qPCR readout for the quantitative measurement of Human SCF R (CD117/c-kit) in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for Human SCF R (CD117/c-kit) coated on a 96-well PCR plate. Standards and samples are pipetted into the wells and SCF R (CD117/c-kit) present in a sample is bound to the wells by the immobilized antibody. The wells are washed and a detection affinity molecule is added to the plates. After washing away unbound detection affinity molecule, primers and a PCR master mix are added to the wells and data is collected using qPCR. Ct values obtained from the qPCR are then used to calculate the amount of antigen contained in each sample, where lower Ct values indicate a higher concentration of antigen.
    Ingrédients
    • SCF R (CD117/c-kit) Microplate (Item A): 96 well PCR plate coated with anti-Human SCF R (CD117/c-kit)
    • Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
    • Standards (Item C): 2 vials of recombinant Human SCF R (CD117/c-kit)
    • Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
    • Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
    • Detection Affinity Reagent for SCF R (CD117/c-kit) (Item F): 2 vials of a 4x concentrated solution of anti-Human SCF R (CD117/c-kit) affinity reagent
    • IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
    • Primer Solution (Item I): 1.7 ml vial
    • PCR Master Mix (Item J): 1.2 ml vial
    • PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
    • Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer
    Matériel non inclus
    • Real-time PCR instrument, Bio-Rad recommended
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • 100 mL and 1 liter graduated cylinders
    • Absorbent paper
    • Distilled or deionized water
    • Log-log graph paper or computer and software for data analysis
    • Tubes to prepare standard or sample dilutions
    • Heating block or water bath capable of 80°C
    Featured
    Discover our best selling KIT Kit ELISA
    Top Product
    Discover our top product KIT Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Commentaires

    Features:
    - Excellent for follow up/confirmation of antibody array data
    - Pre-coated 96 well plate
    - Strip plate format minimizes waste
    - Complete kit includes all necessary reagents
    - 10x more sensitive than ELISA
    - Reproducible and reliable
    - Optimized for serum, plasma, urine and cell culture medium
    - 1/10th the sample volume of an ELISA

    Research Applications:
    - Detection of quantitative protein levels in biological fluids
    - Validation of antibody array results
    - Validation of biomarker discovery studies

    Protocole
    1. Prepare all reagents, samples and standards as instructed
    2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C
    3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
    4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
    5. Add 15μL Primer solution 10μL of PCR master mix to each well
    6. Run real-time PCR
    Restrictions
    For Research Use only
  • Stock
    4 °C,-20 °C,-80 °C
    Stockage commentaire
    May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
    Date de péremption
    6 months
  • Antigène Voir toutes KIT IQ-ELISA Kits
    KIT (Mast/stem Cell Growth Factor Receptor (KIT))
    Autre désignation
    SCF R (KIT Produits)
    Synonymes
    C-Kit Kit IQ-ELISA, CD117 Kit IQ-ELISA, PBT Kit IQ-ELISA, SCFR Kit IQ-ELISA, Bs Kit IQ-ELISA, Fdc Kit IQ-ELISA, Gsfsco1 Kit IQ-ELISA, Gsfsco5 Kit IQ-ELISA, Gsfsow3 Kit IQ-ELISA, SCO1 Kit IQ-ELISA, SCO5 Kit IQ-ELISA, SOW3 Kit IQ-ELISA, Ssm Kit IQ-ELISA, Tr-kit Kit IQ-ELISA, W Kit IQ-ELISA, c-KIT Kit IQ-ELISA, c-kit Kit IQ-ELISA, MGF Kit IQ-ELISA, KIT Kit IQ-ELISA, C-KIT Kit IQ-ELISA, kit Kit IQ-ELISA, receptor Kit IQ-ELISA, sparse Kit IQ-ELISA, Xkl-1 Kit IQ-ELISA, kit-a Kit IQ-ELISA, kit-b Kit IQ-ELISA, kl1-A Kit IQ-ELISA, krk1 Kit IQ-ELISA, pbt Kit IQ-ELISA, scfr Kit IQ-ELISA, KIT proto-oncogene receptor tyrosine kinase Kit IQ-ELISA, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog a Kit IQ-ELISA, KIT proto-oncogene receptor tyrosine kinase S homeolog Kit IQ-ELISA, KIT Kit IQ-ELISA, Kit Kit IQ-ELISA, kita Kit IQ-ELISA, kit Kit IQ-ELISA, kit.S Kit IQ-ELISA
    ID gène
    3815
    UniProt
    P10721
    Pathways
    Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity
Vous êtes ici:
Support technique