Ubiquitin Kit IQ-ELISA

N° du produit ABIN6810523

Détail du produit Ubiquitin Kit IQ-ELISA

Antigène: Ubiquitin

Reactivité: Humain

Méthode de détection: qPCR

Type de méthode: DNA-Binding ELISA

Application: ELISA

Fonction: Human Immunoquantitative (PCR-Based) Ubiquitin+1 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Sensibilité: 3 pg/mL

Attributs du produit: The Immuno Quantitative Enzyme Linked ImumunoSorbent Assay (IQELISA™) is an innovative new assay that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of lower sample volumes while also providing more sensitivity. The Human Ubiquitin+1 IQELISA™ Kit is a modified ELISA assay with high sensitivity qPCR readout for the quantitative measurement of Human Ubiquitin+1 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for Human Ubiquitin+1 coated on a 96-well PCR plate. Standards and samples are pipetted into the wells and Ubiquitin+1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and a detection affinity molecule is added to the plates. After washing away unbound detection affinity molecule, primers and a PCR master mix are added to the wells and data is collected using qPCR. Ct values obtained from the qPCR are then used to calculate the amount of antigen contained in each sample, where lower Ct values indicate a higher concentration of antigen.

Ingrédients:

• Ubiquitin+1 Microplate (Item A): 96 well PCR plate coated with anti-Human Ubiquitin+1
• Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
• Standards (Item C): 2 vials of recombinant Human Ubiquitin+1
• Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
• Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
• Detection Affinity Reagent for Ubiquitin+1 (Item F): 2 vials of a 4x concentrated solution of anti-Human Ubiquitin+1 affinity reagent
• IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
• Primer Solution (Item I): 1.7 ml vial
• PCR Master Mix (Item J): 1.2 ml vial
• PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
• Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer

Matériel non inclus:

• Real-time PCR instrument, Bio-Rad recommended
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for data analysis
• Tubes to prepare standard or sample dilutions
• Heating block or water bath capable of 80^°C

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Features:
- Excellent for follow up/confirmation of antibody array data
- Pre-coated 96 well plate
- Strip plate format minimizes waste
- Complete kit includes all necessary reagents
- 10x more sensitive than ELISA
- Reproducible and reliable
- Optimized for serum, plasma, urine and cell culture medium
- 1/10th the sample volume of an ELISA

Research Applications:
- Detection of quantitative protein levels in biological fluids
- Validation of antibody array results
- Validation of biomarker discovery studies

Protocole:

1. Prepare all reagents, samples and standards as instructed
2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4^°C
3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
5. Add 15μL Primer solution 10μL of PCR master mix to each well
6. Run real-time PCR

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: Ubiquitin

Autre désignation: Ubiquitin (Ubiquitin Produits)

Synonymes: CG11624 Kit IQ-ELISA, DmUb Kit IQ-ELISA, DmUbi-p63E Kit IQ-ELISA, Dmel\\CG11624 Kit IQ-ELISA, Ub Kit IQ-ELISA, Ubi-P63e Kit IQ-ELISA, Ubi-p Kit IQ-ELISA, Ubi63E Kit IQ-ELISA, Ubi63p Kit IQ-ELISA, Ubip Kit IQ-ELISA, Ubiq Kit IQ-ELISA, Ubp Kit IQ-ELISA, ubi Kit IQ-ELISA, ubi-p63E Kit IQ-ELISA, ubi63E Kit IQ-ELISA, CG18282 Kit IQ-ELISA, CG32744 Kit IQ-ELISA, CR32744 Kit IQ-ELISA, DmUbi-p5E Kit IQ-ELISA, Dmel\\CG32744 Kit IQ-ELISA, ubiquitin Kit IQ-ELISA, si:dz180g5.5 Kit IQ-ELISA, si:busm1-180g5.5 Kit IQ-ELISA, LOC100194618 Kit IQ-ELISA, UBIQ Kit IQ-ELISA, Ubiquitin Kit IQ-ELISA, LOC100282068 Kit IQ-ELISA, Ubiquitin-63E Kit IQ-ELISA, Ubiquitin-5E Kit IQ-ELISA, transmembrane and ubiquitin-like domain containing 2 Kit IQ-ELISA, polyubiquitin Kit IQ-ELISA, Putative ubiquitin family protein Kit IQ-ELISA, ubiquitin Kit IQ-ELISA, ubiquitin B Kit IQ-ELISA, UBiQuitin Kit IQ-ELISA, Ubiquitin Kit IQ-ELISA, Ubi-p63E Kit IQ-ELISA, Ubi-p5E Kit IQ-ELISA, tmub2 Kit IQ-ELISA, LOC100194618 Kit IQ-ELISA, pco110026 Kit IQ-ELISA, LOC100135798 Kit IQ-ELISA, UBB Kit IQ-ELISA, ubq-1 Kit IQ-ELISA, LOC100190766 Kit IQ-ELISA

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway