alpha Tubulin Kit ELISA

N° du produit ABIN6810528

Détail du produit alpha Tubulin Kit ELISA

Antigène: alpha Tubulin (TUBA1)

Épitope: acetylated

Reactivité: Humain, Souris, Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human, Mouse, Rat Acetylated-Alpha Tubulin and Alpha Tubulin ELISA Kit. This ELISA is for measuring Acetylated-Alpha Tubulin in Human, Mouse, Rat cell lysates.

Type d'échantillon: Cell Lysate

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes Human///Mouse///Rat Acetylated-Alpha Tubulin.

Attributs du produit:

• Simultaneously measure cleaved protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-cleaved Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: alpha Tubulin (TUBA1)

Autre désignation: Alpha Tubulin (TUBA1 Produits)

Synonymes: Tuba1 Kit ELISA, K-ALPHA-1 Kit ELISA, Tuba-1 Kit ELISA, cb944 Kit ELISA, fb22g06 Kit ELISA, tuba1 Kit ELISA, wu:fb22g06 Kit ELISA, Calpha1 Kit ELISA, tubulin, alpha 1A Kit ELISA, tubulin alpha 1b Kit ELISA, tubulin, alpha 1b Kit ELISA, tubulin, alpha 1B Kit ELISA, Tuba1a Kit ELISA, TUBA1B Kit ELISA, tuba1b Kit ELISA, Tuba1b Kit ELISA

ID gène: 7846

UniProt: Q71U36

Pathways: Dynamique des Microtubules