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COX4NB Kit ELISA

COX4NB Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6951102
  • Antigène Tous les produits COX4NB
    COX4NB (COX4 Neighbor (COX4NB))
    Reactivité
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human EMC8 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Date de péremption
    6 months
  • Antigène Tous les produits COX4NB
    COX4NB (COX4 Neighbor (COX4NB))
    Autre désignation
    EMC8 (COX4NB Produits)
    Synonymes
    COX4NB Kit ELISA, NOC4 Kit ELISA, MGC75783 Kit ELISA, cox4nb Kit ELISA, fb54g02 Kit ELISA, noc4 Kit ELISA, wu:fb54g02 Kit ELISA, zgc:56331 Kit ELISA, C16orf2 Kit ELISA, C16orf4 Kit ELISA, FAM158B Kit ELISA, Cox4nb Kit ELISA, Fam158b Kit ELISA, Noc4 Kit ELISA, ER membrane protein complex subunit 8 Kit ELISA, ER membrane protein complex subunit 8 S homeolog Kit ELISA, ER membrane protein complex subunit 9 Kit ELISA, EMC8 Kit ELISA, emc8 Kit ELISA, emc8.S Kit ELISA, EMC9 Kit ELISA, Emc8 Kit ELISA
    ID gène
    10328
    UniProt
    O43402
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